Interaction of the outer membrane pore-forming protein of Yersinia pseudotuberculosis with lipid a and its synthetic analogs
Fedoreeva, L.I.; Gorbach, V.I.
Bioorganicheskaia Khimiia 19(10): 933-940
1993
ISSN/ISBN: 0132-3423 PMID: 8274168 Document Number: 407153
Interaction of the major outer membrane protein form Yersinia pseudotuberculosis with lipid A was investigated by intrinsic fluorescence, CD spectroscopy and CsCl gradient centrifugation methods. The protein was shown to have two independent binding sites with an association constant 6.1 x 10(4) M-1. The interaction depends on both the type of the glycoside bond and hydrophilic--hydrophobic balance of the glycolipid molecule.
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