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Measurement of the number of single-strand breaks in DNA of irradiated leukemia P-388 using alkaline denaturation

Fomin, V.V.; Borisov, M.B.; Zaĭtsev, A.V.; Blokhin, D.I.

Biulleten' Eksperimental'noi Biologii i Meditsiny 115(4): 416-418

1993

ISSN/ISBN: 0365-9615
PMID: 8049409
Document Number: 406397
Methods, used for indirect determination of single-thread breaks' quantity in DNA, based on it's denaturation in alkali medium and further determination of double-thread DNA quantity by it's fluorescence has been adapted to the measuring of thread disturbances on leukemia P-388 cells. Influence of cells' and alkali concentrations and the time of denaturation of quantity of double-thread DNA has been investigated.

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Leontjeva, G.A.; Mantzighin, Y.A.; Gaziev, A.I. 1976: The ultra-fast repair of single-strand breaks in DNA of gamma-irradiated Chinese hamster cells International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine 30(6): 577-580
Gábelová, A.; Slamenová, D.; Ruzeková, L.; Farkasová, T.; Horváthová, E. 1997: Measurement of DNA strand breakage and DNA repair induced with hydrogen peroxide using single cell gel electrophoresis, alkaline DNA unwinding and alkaline elution of DNA Neoplasma 44(6): 380-388
Erixon, K.; Ahnström, G. 1979: Single-strand breaks in DNA during repair of UV-induced damage in normal human and xeroderma pigmentosum cells as determined by alkaline DNA unwinding and hydroxylapatite chromatography: effects of hydroxyurea, 5-fluorodeoxyuridine and 1-beta-D-arabinofuranosylcytosine on the kinetics of repair Mutation Research 59(2): 257-271
Bryant, P.E.; Blöcher, D. 1982: The effects of 9-beta-D-arabinofuranosyladenine on the repair of DNA strand breaks in X-irradiated Ehrlich ascites tumour cells International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine 42(4): 385-394
Gruzdev, A.D. 1999: Heterochromatin and single-strand DNA breaks (a hypothesis) Genetika 35(7): 869-872
Kulakov, L.A.; Ksenzenko, V.N.; Kochetkov, V.V.; Boronin, A.M. 1987: Localization of canonical single-strand breaks in DNA of the Pseudomonas aeruginosa bacteriophage phi kF77 Molekuliarnaia Biologiia 21(6): 1497-1503
Christensen, R.C.; Tobias, C.A.; Taylor, W.D. 1972: Heavy-ion-induced single- and double-strand breaks in phiX-174 replicative form DNA International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine 22(5): 457-477
Chen, Y.; Goz, B. 1991: Single-strand breaks in DNA by 1-beta-D-arabinofuranosylcytosine and enhanced resistance frequency to N-phosphonoacetyl-L-aspartate Anticancer Research 11(1): 301-304
Lin, W.; Wei, X.; Kelimu, M.; Xue, H.; Lin, H.; Zhang, S.; Zhou, Z. 1999: Studies on DNA single strand breaks induced by sodium nitroprusside-nitric oxide donor Zhonghua Yu Fang Yi Xue Za Zhi 33(6): 360-362
Coleman, J.B.; Gilfor, D.; Farber, J.L. 1989: Dissociation of the accumulation of single-strand breaks in DNA from the killing of cultured hepatocytes by an oxidative stress Molecular Pharmacology 36(1): 193-200
Singh, D.R.; Nair, C.K.; Pradhan, D.S. 1991: Chemical radiosensitization by misonidazole: production and repair of DNA single-strand breaks in Yoshida ascites tumor cells Indian Journal of Experimental Biology 29(7): 601-604
Bradley, M.O.; Dysart, G.; Fitzsimmons, K.; Harbach, P.; Lewin, J.; Wolf, G. 1982: Measurements by filter elution of DNA single- and double-strand breaks in rat hepatocytes: effects of nitrosamines and gamma-irradiation Cancer Research 42(7): 2592-2597
Lubet, R.A.; Kiss, E.; Gallagher, M.M.; Dively, C.; Kouri, R.E.; Schechtman, L.M. 1983: Induction of neoplastic transformation and DNA single-strand breaks in C3H/10T1/2 clone 8 cells by polycyclic hydrocarbons and alkylating agents Journal of the National Cancer Institute 71(5): 991-997
Vexler, F.B.; Kublik, L.N.; Eidus, L.Kh.; Vexler, A.M. 1982: Kinetics of repair of DNA single-strand breaks in cultured mammalian cells. Part I. The dependence on irradiation dose and cell cycle stage International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine 41(4): 453-457
Blöcher, D. 1982: DNA double strand breaks in Ehrlich ascites tumour cells at low doses of x-rays. I. Determination of induced breaks by centrifugation at reduced speed International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine 42(3): 317-328
Kulkarni, M.S.; Cox, B.A.; Yielding, K.L. 1982: Requirements for induction of DNA strand breaks by lithocholic acid Cancer Research 42(7): 2792-2795
Dorr, R.T.; Bellamy, W.; Liddil, J.D.; Baker, A.; Bair, K.W. 1998: Correlation of cytotoxicity and protein-associated DNA strand breaks for 2-(arylmethylamino)-1,3-propanediols Anti-Cancer Drug Design 13(7): 825-835
Rezácová, M.; Vávrová, J.; Cerman, J. 2005: A cell and genotoxic stress: a reaction to double strand breaks of DNA Casopis Lekaru Ceskych 144(Suppl 3): 13-17
Burilkov, V.K.; Kovarskiĭ, E.V.; Krochik, G.M. 1988: Induction of DNA strand breaks by laser irradiation in a visible range Biofizika 33(2): 242-246
Piette, J. 1979: Guanine alteration and production of strand breaks in DNA photosensitized by proflavine Archives Internationales de Physiologie et de Biochimie 87(5): 1050-1051