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DNA double strand breaks in Ehrlich ascites tumour cells at low doses of x-rays. I. Determination of induced breaks by centrifugation at reduced speed

Blöcher, D.

International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine 42(3): 317-328

1982

ISSN/ISBN: 0020-7616
PMID: 6982882
Document Number: 186232
DNA double strand breaks (dsb) were determined in Ehrlich . The method is based on the separation of DNA from other components by heating in a solution of pronase and detergents held in wide-mouth syringes, which were also used facilitate the application of the released high-MW DNA to sucrose gradients. Purified DNA was sedimented in neutral sucrose gradients at low speed to reduce speed artifacts. The sedimentation profiles were analyzed using a computer program and the number of dsb was determined by simulation of random breaks in the mass distribution of the control sample and by comparison of this simulated profile with that of the irradiated one. The number of dsb formed was proportional to X-ray dose in the range of 5-2000 Gy.

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