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Detection of DNA strand breaks induced by hydroxyl radicals in nuclear and chromosomal chromatin by electron microscopy

Fernández, J.L.; Gosálvez, J.; Goyanes, V.J.

Cytobios 73(294-295): 189-195

1993

ISSN/ISBN: 0011-4529
PMID: 8500350
Document Number: 411340
Chinese hamster Don cells were treated with 10 mM hydrogen peroxide. DNA strand breaks induced by hydroxyl radicals were amplified in 3' termini by an exonuclease III digestion, resulting in single stranded DNA motifs. In situ detection of these motifs was performed on chromatin fibres of isolated whole-mounted nuclei and chromosomes by a random priming procedure, using biotinylated-dUTP which bound a gold-tagged streptavidin. This approach facilitated the location of hydroxyl radical induced DNA breaks, specifically on 20-30 nm diameter chromatin fibres, by transmission electron microscopy.

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Maehara, Y.; Anai, H.; Kusumoto, T.; Sakaguchi, Y.; Emi, Y.; Kohnoe, S.; Sugimachi, K. 1989: Detection of cellular DNA strand breaks induced by antitumor drug and heat using in situ nick translation Nihon Geka Gakkai Zasshi 90(9): 1576-1578
Elia, M.C.; Bradley, M.O. 1992: Influence of chromatin structure on the induction of DNA double strand breaks by ionizing radiation Cancer Research 52(6): 1580-1586
Blöcher, D. 1982: DNA double strand breaks in Ehrlich ascites tumour cells at low doses of x-rays. I. Determination of induced breaks by centrifugation at reduced speed International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine 42(3): 317-328
Miyake, M.; Fuchimoto, S.; Iwagaki, H.; Matsubara, N.; Edamatsu, R.; Hiramatsu, M.; Orita, K. 1991: Production of hydroxyl radicals by tumor cells varies with cell type as measured by electron spin resonance spectrometry Research Communications in Chemical Pathology and Pharmacology 71(3): 293-307
Kamesaki, S.; Kamesaki, H.; Jorgensen, T.J.; Tanizawa, A.; Pommier, Y.; Cossman, J. 1993: bcl-2 protein inhibits etoposide-induced apoptosis through its effects on events subsequent to topoisomerase II-induced DNA strand breaks and their repair Cancer Research 53(18): 4251-4256
Ludwig, C.U.; Bowden, G.T.; Roberts, R.A.; Alberts, D.S. 1984: Reduced bisantrene-induced cytotoxicity and protein-associated DNA strand breaks under hypoxic condition Cancer Treatment Reports 68(2): 367-372
Christensen, R.C.; Tobias, C.A.; Taylor, W.D. 1972: Heavy-ion-induced single- and double-strand breaks in phiX-174 replicative form DNA International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine 22(5): 457-477
Lin, W.; Wei, X.; Kelimu, M.; Xue, H.; Lin, H.; Zhang, S.; Zhou, Z. 1999: Studies on DNA single strand breaks induced by sodium nitroprusside-nitric oxide donor Zhonghua Yu Fang Yi Xue Za Zhi 33(6): 360-362
Gaginskaia, E.R.; Tsvetkov, A.G. 1988: Electron microscopy research on the chromatin structure of dispersed lampbrush chromosomes in the hen Tsitologiia 30(2): 142-150
Ávalos, A.; Haza, A.I.; Morales, P. 2015: Manufactured silver nanoparticles of different sizes induced DNA strand breaks and oxidative DNA damage in hepatoma and leukaemia cells and in dermal and pulmonary fibroblasts Folia Biologica 61(1): 33-42
Kuzhir, T.D.; Dalivelia, O.V. 1993: The effect of antioxidants on the formation of ethylmethane sulfonate-induced chromosomal breaks in relation to the maternal repair systems in Drosophila melanogaster Vestnik Rossiiskoi Akademii Meditsinskikh Nauk 1: 56-64
Erixon, K.; Ahnström, G. 1979: Single-strand breaks in DNA during repair of UV-induced damage in normal human and xeroderma pigmentosum cells as determined by alkaline DNA unwinding and hydroxylapatite chromatography: effects of hydroxyurea, 5-fluorodeoxyuridine and 1-beta-D-arabinofuranosylcytosine on the kinetics of repair Mutation Research 59(2): 257-271
Buck, R.C. 1973: Walker 256 tumor implantation in normal and injured peritoneum studied by electron microscopy, scanning electron microscopy, and autoradiography Cancer Research 33(12): 3181-3188
Bouillard, C.; Miegeville, M.; Vermeil, C. 1983: Use of scanning electron microscopy coupled with transmission electron microscopy in comparative studies of the relation between Schistosoma mansoni and Salmonella typhimurium Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 177(2): 149-157
Marin, M.L.; Geller, S.A.; Greenstein, A.J.; Marin, R.H.; Gordon, R.E.; Aufses, A.H. 1983: Ultrastructural pathology of Crohn's disease: correlated transmission electron microscopy, scanning electron microscopy, and freeze fracture studies American Journal of Gastroenterology 78(6): 355-364
Hang, Z.B.; Wei, Y.Q. 1989: Application of electron microscopy to tumor diagnosis--observation of 1000 cases with both light and electron microscopy Hua Xi Yi Ke da Xue Xue Bao 20(2): 165-169
Gruzdev, A.D. 1999: Heterochromatin and single-strand DNA breaks (a hypothesis) Genetika 35(7): 869-872
Kulkarni, M.S.; Cox, B.A.; Yielding, K.L. 1982: Requirements for induction of DNA strand breaks by lithocholic acid Cancer Research 42(7): 2792-2795
Dorr, R.T.; Bellamy, W.; Liddil, J.D.; Baker, A.; Bair, K.W. 1998: Correlation of cytotoxicity and protein-associated DNA strand breaks for 2-(arylmethylamino)-1,3-propanediols Anti-Cancer Drug Design 13(7): 825-835
Burilkov, V.K.; Kovarskiĭ, E.V.; Krochik, G.M. 1988: Induction of DNA strand breaks by laser irradiation in a visible range Biofizika 33(2): 242-246