Contents of extracellular DNA in blood of irradiated rats
Vladimirov, V.G.; Belokhvostov, A.S.; Sherlina, S.S.; Vasil'eva, I.N.; Voskresenskiĭ, A.M.
Biulleten' Eksperimental'noi Biologii i Meditsiny 113(2): 188-191
1992
ISSN/ISBN: 0365-9615 PMID: 1611072 Document Number: 397776
Intact rat plasma contains high-molecular DNA which moves as a single fraction in 0.5% agarose electrophoresis. As early as 2-5 hours after gamma-irradiation in a dose of 1-100 Gy there appears low-molecular DNA (about 180 nucleotide pairs), the amount of which directly correlates with the irradiation dose 5 hours after the exposure. Blot-hybridization showed that low-molecular DNA has no common nucleotide sequences with high-molecular DNA, though has sites similar to genome repeat sequences.