Bile salts promote adherence-decreasing effect of colonic luminal hydrolases on Entamoeba histolytica
Variyam, E.P.
Archives of Medical Research 23(2): 223-225
1992
ISSN/ISBN: 0188-4409 PMID: 1340299 Document Number: 395896
Trophozoites of Entamoeba histolytica cultivated for > 7 hours in the presence of glycosidases, produced by a subset of the colonic anaerobic bacteria of healthy humans, and pancreatic proteases show decreased adherence to Chinese hamster ovary (CHO) epithelial cells. Since bile salts enhance the activities of glycosidases, they were investigated to see whether they would enhance the E. histolytica-CHO cell adherence decreasing effects of the luminal hydrolases (glycosidases plus proteases). CHO cell adherence of control trophozoites was 78.4 +or- 1.2%. Incubation with hydrolases alone for 4 hours did not change adherence. When sodium taurocholate was added to the hydrolases for 4 hours, trophozoite adherence dropped to 30.25 +or- 3.2% of the control level. Incubation with sodium taurodeoxycholate at 2.0 and 5.0 mM also decreased trophozoite adherence to 25.1 +or- 2.9% and 29.4 +or- 1.7%, respectively. These findings show that bile salts enhance the effects of luminal hydrolases on E. histolytica trophozoites, decreasing their ability to adhere to epithelial cells.