Axenic massive cultivation of Entamoeba histolytica trophozoites in spinner flasks and in a microfermentor
Said-Fernández, S.; Mata-Cárdenas, B.D.; Castro-Garza, J.; Martínez-Rodríguez, H.; Navarro-Marmolejo, L.; Lozano-Garza, G.
Archives of Medical Research 23(2): 57-58
1992
ISSN/ISBN: 0188-4409 PMID: 1340322 Document Number: 395183
A medium, PEHPS, whose main components are extracts of ox liver, and ox and swine pancreas was used to develop two alternative methods for axenic cultivation of trophozoites of Entamoeba histolytica in suspension. Firstly, in spinner culture flasks with a magnetic stirrer which gave yields of 2.36 x 105 to 3.08 x 105 and secondly, in a 14 litre capacity microfermenter, which produced 1.65 x 105 amoebae/ml in one batch and 2.94 to 3.65 x 105 amoebae/ml at semicontinuous flux.