Amino acid composition in total proteins of strains of Entamoeba histolytica in axenic cultures
López-Revilla, R.; Navarro, V.
Archivos de Investigacion Medica 9(Suppl 1): 129-132
1978
ISSN/ISBN: 0066-6769 PMID: 211943 Document Number: 134687
In this paper we detail the amino acid composition of total protein hydrolyzates from trophozoites of two Entamoeba histolytica strains (HK-9:NIH and HM-2:IMSS) cultured axenically in TP-S-1 medium. Since we obtained nearly identical results in both strains, amino acid patterns may constitute an additional taxonomic criterion of the species. Our data are strikingly different from those found previously by other workers with total proteins from HK-9:NIH trophozoites cultured in a bacteria-conditioned medium; such difference emphasize the importance of axenic culture for the precise study of the chemical composition and metabolism of E. histolytica. Amino acid composition of total trophozoite proteins could be of use to attempt the design of a culture medium of defined amino acid content.