A method for studying the three-dimensional structure of organs and tissue in pathology
Rekhter, M.D.; Kolpakov, V.A.; Mironov, A.A.
Arkhiv Patologii 54(1): 65-68
1992
ISSN/ISBN: 0004-1955 PMID: 1596189 Document Number: 389741
A new technique of scanning electron microscopy for visualization of hidden cellular surfaces in organs and tissue has been developed. It employs the sample incubation in 30% potassium hydroxide and 96% alcohol mixed in equal amounts and dissociation until single cells appear in the solution, this being checked under phase-contrast microscope. The method is beneficial in non-standard cases of three-dimensional organization of organs and tissues.
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