Growth inhibition of N1E-115 mouse neuroblastoma cells by c-myc or N-myc antisense oligodeoxynucleotides causes limited differentiation but is not coupled to neurite formation
Larcher, J.C.; Basseville, M.; Vayssiere, J.L.; Cordeau-Lossouarn, L.; Croizat, B.; Gros, F.
Biochemical and Biophysical Research Communications 185(3): 915-924
1992
ISSN/ISBN: 0006-291X PMID: 1627144 Document Number: 389329
Antisense oligodeoxynucleotides were found to be stable in the culture medium containing fetal calf serum (heat-activated 30 minutes at 65.degree.C) and in cells. Antisense oligomer treatment causes cessation of mitoses, but does not lead to morphological differentiation. Under antisense conditions, we have observed an increase in the amount of two neurospecific protein, namely peripherin and gamma-enolase. Comparison of the results obtained with chemical inducers and antisense olideoxynucleotides allows us to postulate three phases in N1E-115 differentiation: the first correspond to the arrest of mitosis, the second to the expression of a limited neuronal program, and the third to the morphological and electrophysiological differentiation.