Inhibition of antisense VEGF gene on the growth of glioma cells BT325
Sun, L.; Lu, J.; Gong, W.; Liu, Y.; Han, F.; Wang, Q.; Zhang, S.; Huang, Z.
Wei Sheng Yan Jiu 44(3): 413-416
2015
ISSN/ISBN: 1000-8020 PMID: 26137620 Document Number: 680236
To investigate the inhibitory effect of antisense VEGF gene on growth of human glioma BT325 and induced apoptosis in cells. The pcDNA3.1/anti-VEGF eukaryotic expression vector was constructed and transfected into human glioma BT325. The ELISA assay was used to assess the expression of VEGF gene. Soft agar colony formation, MTT assay and electron microscope were used to evaluate the proliferation, apoptosis and the morphological changes of BT325. Compared with a control group, the level of VEGF expression was significantly decreased and was almost completely suppressed. The amount in soft agar colony at vector group and antisense VEGF gene group were 21 and 2, respectively. The growth of BT325 was significantly inhibited to 38.23% and resulted in the apoptotic morphology by antisense VEGF gene under electron microscope, compared to vector group. Antisense VEGF gene inhibited the growth and proliferation, induced cell apoptosis, played an efficient role of anti-cancer in BT325 cells.