Inhibition of reverse transcription by unmodified and modified antisense oligodeoxynucleotides
Boiziau, C.; Blonski, C.; Thuong, N.T.; Shire, D.; Toulme, J.J.
Nucleic Acids Symposium Series 24: 121-125
1991
ISSN/ISBN: 0261-3166 PMID: 1726738 Document Number: 385336
We used oligodeoxynucleotides to prevent reverse transcription of beta-globin mRNA by reverse transcriptase of avian myeloblastosis virus. Unmodified oligomers hybridized to the template arrested synthesis of cDNA in a dose dependent manner. The longer the oligomer the more efficient the inhibition, 50% inhibition being achieved at 0.3 and 30 microM of a 17- or a 12-mer, respectively. The use of complementary oligonucleotides with a 3' end blocked either by a dideoxy residue or by a dodecanol group also induced inhibition of cDNA synthesis.