Respiratory activity of isolated hepatocytes
Petrenko, A.Iu.
Ukrainskii Biokhimicheskii Zhurnal 63(4): 114-118
1991
ISSN/ISBN: 0201-8470 PMID: 1949225 Document Number: 387021
Oligomycin and uncoupler of oxidative phosphorylation have been studied for their effect on the respiration activity of hepatocytes in rats. The respiration rate in the presence of oligomycin and uncoupler is higher than it is with the respiration upcoupled in the absence of oligomycin. Exogenic succinate makes endogenic respiration of hepatocytes in the presence of digitonin 5 times more intensive. The obtained results evidence for the fact that the uncoupled respiration is limited by the concentration of substrates able to be oxidized in the respiration chain of mitochondria. Oligomycin induces accumulation of substrates and following addition of the disconnector evokes their fast oxidation.