Xenobiotic and biochemical parameters of isolated rat hepatocytes
Popović, M.; Daković-Svajcer, K.; Nerudova, J.
Arhiv za Higijenu Rada i Toksikologiju 43(1): 21-27
1992
ISSN/ISBN: 0004-1254 PMID: 1510613 Document Number: 397573
The authors investigated the effects of the inductors (griseofulvin and fenitoin) and the inhibitors of the cytochrome P-450 (metronidazole, chloramphenicol and phenylbutazone), the effects of the drugs with the purine structure (aminophylline, xanthiol-nicotinate, pentoxiphylline, azothioprine, thioguanine, 6-mercaptopurine), and the effects of several pesticides (lindane, binapicrile, parathion) on some biochemical parameters of the rat liver. The following parameters were determined: viability of hepatocytes, the content of glutathione and cytochrome P-450, and the activity of xanthinoxidase and lipoperoxidase. The experiments were performed in vivo and in vitro. The results showed that the cell viability as well as the other parameters studied, were most drastically affected by chloramphenicol, azathioprine and parathion, whereas the other substances elicited less intensive changes.