Doxapram inhibits the in vitro oxidation of hexobarbital
Ishikawa, M.; Ozaki, M.; Takayanagi, Y.; Sasaki, K.
Research Communications in Chemical Pathology and Pharmacology 72(3): 371-374
1991
ISSN/ISBN: 0034-5164 PMID: 1719592 Document Number: 385692
The effect of doxapram on the mouse liver microsomal hexobarbital metabolism in vitro was studied. Doxapram inhibited hexobarbital oxidase in a competitive manner, with inhibition constants between 2 and 10 mM. Doxapram induced a reverse type I spectral change with a spectral dissociation constant of about 0.1 microM. These results indicate that doxaprame is an inhibitor of microsomal drug metabolism in the mouse.