Isolation and axenization of Giardia lamblia isolates from symptomatic and asymptomatic patients in Mexico
Cedillo-Rivera, R.; Enciso-Moreno, J.A.; Martínez-Palomo, A.; Ortega Pierres, G.
Archivos de Investigacion Medica 22(1): 79-85
1991
ISSN/ISBN: 0066-6769 PMID: 1819980 Document Number: 380320
Giardia lamblia [Giardia duodenalis] isolates from faeces of 5 symptomatic and 14 asymptomatic children from Mexico City, collected between September 1986 and June 1987, were cultured under axenic conditions. The isolation procedure was a modification of available methods and involved: concentration and cleaning of cysts by centrifugation in sucrose gradients; excystment induction in acid solution; culture in modified TYI-S-33 medium and axenization of isolates using ceftriaxone and amphotericin B. Isolates from symptomatic and asymptomatic cases were equally amenable to isolation and axenic culture.