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Electron microscopic localization of F-actin in acrosome reacted boar spermatozoa by means of a phalloidin-FITC complex

Castellani-Ceresa, L.; Brivio, M.F.; Radaelli, G.

Journal of Submicroscopic Cytology and Pathology 23(2): 347-349

1991

ISSN/ISBN: 1122-9497
PMID: 1906373
Document Number: 380120
An immunocytochemical study at the ultrastructural level has been performed in boar spermatozoa in order to clarify the aggregation state of actin before and after the acrosome reaction. A new phalloidin probe has been used to detect F-actin: a phalloidin derivative conjugated with FITC, followed by incubation with an anti-FITC antibody. The protein A-gold technique was then applied for the localization of the antigenic sites. Gold particles were localized on the sperm surface only after the acrosome reaction which was induced by the ionophore A23187.

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