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Increase in the quantity of highly repetitive sequences in extrachromosomal circular DNAs under inhibition of translation by cycloheximide

Krokhina, T.B.; Raevskaia, G.B.; Chernyĭ, D.I.; Gar'kavtsev, I.V.

Biulleten' Eksperimental'noi Biologii i Meditsiny 111(5): 530-532

1991

ISSN/ISBN: 0365-9615
PMID: 1878571
Document Number: 377663
Small polydispersed circular DNA(spcDNA) was isolated from cultivated HeLa cells. Cells were treated by cycloheximide in concentrations of 1 and 50 micrograms/ml. Gradient fractions were dot blotted to nitrocellulose filters and were hybridized with different repetitive DNAs. The pool of repetitive DNA sequences in fraction of spcDNA increased for cycloheximide treated cells. The content of Alu sequences increased by 1.5-2.5 times, "classical" satellite DNA--by 5-7 times, alpha-satellite--by 3-5 times.

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