Isolation and characteristics of 2 transcribing families of pigeon-genome repetitive sequences
Gazarian, K.G.; Kuznetsova, E.D.; Tarantul, V.Z.; Zaboĭkina, T.N.
Molekuliarnaia Biologiia 11(5): 1010-1021
1977
ISSN/ISBN: 0026-8984 PMID: 618335 Document Number: 116125
From pigeon DNA two families of repeats have been isolated: one (frequency of repetitions 30--40 times per haploid genome) actively transcribing and the second (frequency of repetitions 2000--2800 times) weakly transcribing in erythroid cells. The reassociation kinetics, GC-content and size of isolated repeats were investigated. Actively transcribing repeats (average length of 350 nucleotides) contain about 55% GC-nucleotides and alternate in the genome with AT-rich sequences of the unique type. Comparison of isolated families of repeats revealed differences in the structure and posttranscriptional fate of RNA transcribed from them.