The effect of ethanol on increased expression of HLA-A,-B antigens on lymphocytes
Májský, A.
Vnitrni Lekarstvi 37(9-10): 789-794
1991
ISSN/ISBN: 0042-773X PMID: 1771813 Document Number: 374259
Ethanol in a concentration of 0.001% to 2% increases the expressivity of HLA-A, B antigens on lymphocytes. In 26.13% of the examinations HLA antigens were proved only on lymphocytes processed with ethanol. The expressivity is maximally enhanced already by short-term incubation of lymphocytes with ethanol; if prolonged to 120 mins at 20 degrees C ethanol does not produce an unspecific positivity of results. There is no difference between processing of lymphocytes at 20 degrees C and 37 degrees C. 1% and 2% ethanol facilitates detection of HLA-A, B antigens after addition to serum and lymphocytes in the first stage of the microlymphocytotoxic test.