A simple method for estimation of phospholipids in biological objects
Lemeshko, V.V.; Brovkovich, V.M.; Listopad, A.P.
Ukrainskii Biokhimicheskii Zhurnal 63(1): 60-66
1991
ISSN/ISBN: 0201-8470 PMID: 2028518 Document Number: 373849
A simple method to estimate phospholipids is elaborated. This method is based on determination of optical density of phospholipid-molibdate complexes in chloroform. The method is used for the quantitative determination of phospholipids in biomembranes, liposomes and blood serum without their preliminary extraction, as well as in chloroform, methanol and chloroform-methanol solutions. It is also modified for the phospholipids determination in chromatographic fractions on silufol plates.