Introduction of a high-resolution cytochemical method for studying the distribution of phospholipids in biological tissues
Coulombe, P.A.; Kan, F.W.; Bendayan, M.
European Journal of Cell Biology 46(3): 564-576
1988
ISSN/ISBN: 0171-9335 PMID: 3181170 Document Number: 314288
The method described is based on enzyme-gold cytochemistry. Phospholipase A2 (PLA2) from honeybee venom is adsorbed on gold particles and used for the specific labelling of its substrate, sn3-glycerophospholipids. This report describes the preparation of the PLA2-gold complex and the conditions required for in situ labelling of phospholipids.
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