A simple method for quantitative estimation of collagen type IIi to type i ratio in soft tissues
Cetta, G.; Tenni, R.; Castellani, A.A.
Italian Journal of Biochemistry 28(3): 163-172
1979
ISSN/ISBN: 0021-2938 PMID: 553900 Document Number: 143359
In the present paper a relatively rapid and simple method for estimating the ratio of collagen type III to type I in soft tissues is proposed. The ratio Gly/Ala is determined in pure collagen samples obtained from pepsin digests of the tissues. Since this ratio varies linearly depending on the composition of the mixtures of the two collagen types, it is shown that the percentage content of the two collagen types is easily calculated.
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