A simple procedure for the purification of neutral salt soluble type I collagen from skin
Speranza, M.L.; Valentini, G.
Italian Journal of Biochemistry 35(1): 42-48
1986
ISSN/ISBN: 0021-2938 PMID: 3710766 Document Number: 335
Intact monomeric type I collagen was purified from fetal bovine skin by a simple and time saving procedure. Saline precipitates of mixed skin collagens, in 4 M NaCl, were extracted with a limited volume of dilute acetic acid, taken in the proportion of 1 ml per g of original wet skin; NaCl in the precipitate was not removed by dialysis. The salt concentration in the extraction medium in the above conditions, selectively solubilized type I collagen.
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