In situ transcription and determination of mRNA in human thymic antigen 1a positive cells within epidermis
Ding, T.; Kolenik, S.A.; Longley, J.; Edelson, R.L.
Zhonghua Yi Xue Za Zhi 71(1): 6-7; 2
1991
ISSN/ISBN: 0376-2491 PMID: 1708688 Document Number: 369364
This paper introduces a method of study on the characteristics of cell surface protein. We used human thymic antigen 1a anti-sense oligonucleotide primer, radiolabeled deoxynucleotide and in situ transcription with cells. The results of in situ transcription and determination of mRNA in CD1a positive cells isolated from the epidermis of the prepuce of normal neonate showed that most of the positive cells contained specific CD1a mRNA, and the specificity and sensitivity of this method were higher and the procedure was relatively simple.