Detection of Ki-ras proto-oncogene protein by a specific monoclonal antibody
Palejawala, S.; Goldsmith, L.T.
Biotechniques 11(5): 606-608
1991
ISSN/ISBN: 0736-6205 PMID: 1804249 Document Number: 369365
We have used a commercially available mouse monoclonal antibody and shown it to bind specifically to cellular Ki-ras p21 proteins and not to cellular N- and Ha-ras p21 proteins. In conjunction with electrophoresis and Western blotting, this antibody can be used, with further detailing, to assess levels of the cellular Ki-ras p21 against a background of total p21s.
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