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Detection of mRNA in cultured cells transfected with recombinant DNA by in situ hybridization

Ikeda, S.; Oda, T.

Cellular and Molecular Biology 36(5): 601-607

1990

ISSN/ISBN: 0145-5680
PMID: 2073684
Document Number: 352322
Neomycin resistant mRNA stably expressed in pSV2neo transformed cells was positively stained in the cytoplasm of the cells by in situ hybridization with a biotinylated probe. A molecularly cloned provirus genome of a retrovirus produced in a human lymphoblastoid cell line was introduced into canine fetal thymus cells (a host cell for the virus), and then the transient expressions of viral antigen and RNA were detected in the transfected cells by immunoperoxidase staining and in situ hybridization with biotin- or 32P-labelled probes.

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