Ia antigens are expressed on ATPase-positive dendritic cells in chicken epidermis
Pérez Torres, A.; Millan Aldaco, D.A.
Journal of Anatomy 184: 591-596
1994
ISSN/ISBN: 0021-8782 PMID: 7928646 Document Number: 422717
Langerhans cells (LC) are antigen-presenting dendritic cells located in mammalian epidermis and in other stratified epithelia. We recently demonstrated the presence of Langerhans-like cells in the epidermis of the chicken using ultrastructural histochemistry for adenosine triphosphatase (ATPase). The aim of the present study was to test whether ATPase-positive dendritic cells also express class II histocompatibility molecules (Ia antigens) encoded by the major histocompatibility complex (MHC), using a double staining technique, in separated chicken epidermal sheets. We concluded that the epidermal dendritic cells observed are the LC of the chicken, based on their morphology and spatial distribution, but mainly on the complete overlap for ATPase reaction and Ia antigen expression, these being reliable markers for the identification of mammalian LC.