Structural changes in the sarcoplasmic reticulum membrane of skeletal muscles at the early stage of x-ray irradiation
Khizhniak, S.V.; Voĭtsitskiĭ, V.M.; Kucherenko, N.E.
Ukrainskii Biokhimicheskii Zhurnal 63(3): 113-117
1991
ISSN/ISBN: 0201-8470 PMID: 1833869 Document Number: 368804
A single X-ray irradiation of the rabbit hindlimbs in a dose of 0.24 C/kg evokes a decrease in fluorescence of the ANS probe bound with membranes of the sarcoplasmatic reticulum as a result of the decrease of binding sites, binding constant as well as the quantum output of the probe. A decrease in fluorescence of tryptophan residues of Ca-ATPase localized in membranes and attenuation of interaction of its SH-group with dithionitrobenzoic acid has been also observed at early postradiation terms (1 and 24 h). The obtained results evidence for structural rearrangements occurring in membranes of the sarcoplasmic reticulum under the effect of ionizing radiation. Changes in conformation of CA-ATPase molecules contribute much to this process.