The use of the annihilated delayed fluorescence phenomenon in the study of the structural organization of the sarcoplasmic reticulum
Mekler, V.M.; Kotel'nikov, A.I.; Likhtenshteĭn, G.I.; Kotel'nikova, R.A.; Tat'ianenko, L.V.
Biofizika 29(5): 809-813
1984
ISSN/ISBN: 0006-3029 PMID: 6509099 Document Number: 225945
Self-interaction of triplet-excited probes and interaction between the triplet-excited probes and a quencher (ferrocene) at 30 degrees C have been studied in Sarcoplasmic reticulum membranes (SR) and in dimiristoyl phosphatidylcholine liposomes by means of annihilated delayed fluorescence (ADF) registration. Perylene, 7,12-dimethyl benzanthracene and 4-(2-anthryl) butanoic acid have been used as triplet probes. An average time decay ADF of perylene and 4-(2-anthryl) butanoic acid in SR is equal to 4,2: 10(-5) s and 2,3 X 10(-5) s, respectively. Triplet states constant quenching of perylene by ferrocene is equal to 2,1 X 10(7) (mM/g of lipids)-1 X s-1 in SR and 4,5 X 10(7) (mM/g of lipids)-1 X s-1 in liposomes. This means that lipids in a liquid crystalline phase form continuous extended regions containing more than 7 X 10(3) lipid molecules and free of significant hindrances in probes diffusion.