Intestinal gradient of enterokinase activity in different species of animals

Malis, F.; Lojda, Z.; Stépánková, R.; Slezák, Z.

Acta Universitatis Carolinae. Medica. Monographia 1977(77 Part 1): 113-117

1977


ISSN/ISBN: 0567-8250
PMID: 97929
Document Number: 3648
Enterokinase (enteropeptidase, EC 3.4.4.8.) is the key enzyme in the intraluminal digestion of proteins in the small intestine, as it converts trypsinogen by proteolysis to trypsin, which then activates the other pancreatic proteolytic proenzymes, i, e. chymotrypsinogens, procarboxypeptidases and proelastase. In an earlier study (3) we showed that enterokinase was localized in the enterocyte brush border of the duodenum and that its activity diminished distinctly in an aboral direction. Cases of enterokinase deficiency were recently described, including children (1, 2, 5), and the signs were identical with those of trypsin deficiency. Diminished enterokinase activity in man evidently causes an impaired coordination of digestive processes. To obtain more detailed information on enterokinase problems we studied the proximodistal intestinal gradient of this enzyme in various species of animals and in man, its activity in gnotobiological rats and, lastly, the methodological question of whether, after a given degree of solubilization, the determination of enterokinase activity in the supernatant could be used.

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Intestinal gradient of enterokinase activity in different species of animals