Immunoelectrophoresis of mycobacterial antigens
Radhakrishnan, V.V.; Mathai, A.; Rao, S.B.; Sehgal, S.
Indian Journal of Experimental Biology 28(9): 812-815
1990
ISSN/ISBN: 0019-5189 PMID: 2126254 Document Number: 363373
Polyvalent antiserum to culture filtrate of H37 Ra Mycobacterium tuberculosus was raised in rabbits. Monospecific antiserum was raised against M. tuberculosis antigen-5, prepared from the culture filtrates by immunoabsorbent affinity chromatography. On immunoelectrophoresis, antigen-5 demonstrated single precipitin arc against polyvalent and monospecific antisera. The culture filtrate antigen demonstrated multiple precipitin arcs against polyvalent antiserum and single precipitin arc against monospecific antiserum. Antigen-5 could be isolated and characterized from the culture filtrate of H37 Ra M. tuberculosis. Immunoelectrophoresis could be one of the method to characterize the mycobacterial antigens prepared in the laboratory.