Induction of retarded exoerythrocytic schizonts by chloroguanide resulting in delayed parasitaemia of Plasmodium inui in Macaca mulatta
Jiang, J.B.; Liao, J.Y.; Huang, J.C.; Liang, D.S.; Li, D.S.; Lun, Z.R.
Zhongguo Yao Li Xue Bao 11(3): 272-274
1990
ISSN/ISBN: 0253-9756 PMID: 2088005 Document Number: 361187
Three rhesus monkeys inoculated with a large number of sporozoites of Plasmodium inui dissected from the salivary glands of infected mosquitoes, Anopheles dirus. Two of the monkeys (inoculated with 8.06 .times. 105 and 1.3 .times. 107 sporozoites, respectively) were treated with chloroguanide base 6 mg/(kg .cntdot. d) .times. 4 d starting from 24 h after the inoculations and the other one (inoculated with 5.93 .times. 106 sporozoites) was not treated with chloroguanide as control. The primary parasitaemia attacks occurred in the former 2 monkeys were 31 and 25 d respectively after inoculation, while in the control was 7.5 d. Liver biopsies were done in all of the 3 monkeys, normal schizonts were seen in the control monkey on d 8 after inoculation, while none were detected in the 2 monkeys treated with chloroguanide. However, the retarded exoerythrocytic schizonts were found by indirect fluorescent antibody test (IFAT) in liver sections of the monkey inoculated with 1.3 .times. 107 sporozoites. Therefore, it is evident that moderate doses of chloroguandie retarded the formation of schizonts and thus delayed the primary parasitaemia.