In vitro cultivation of exoerythrocytic stages of the human malaria parasite Plasmodium malariae
Millet, P.; Collins, W.E.; Fisk, T.L.; Nguyen-Dinh, P.
American Journal of Tropical Medicine and Hygiene 38(3): 470-473
1988
ISSN/ISBN: 0002-9637 PMID: 3079312 Document Number: 317682
Exoerythrocytic stages of P. malariae (Uganda-I/CDC strain) were obtained in vitro by inoculating primary cultures of hepatocytes from a chimpanzee (Pan troglodytes) and a monkey (Aotus lemurinus griseimembra) with sporozoites from artificially infected Anopheles stephensi salivary glands. Schizonts were observed in chimpanzee hepatocytes 8, 11 and 13 days after inoculation. Only one schizont was seen in Aotus hepatocytes at day 13. The morphology and development rates of P. malariae exoerythrocytic stages obtained in vitro were similar to those previously described in vivo. Exoerythrocytic stage parasites of Plasmodium malariae were obtained in vitro by inoculating primary cultures of hepatocytes from a chimpanzee (Pan troglodytes) and a monkey (Aotus lemurinus griseimembra) with sporozoites. Schizonts were observed in chimpanzee hepatocytes 8, 11, and 13 days after inoculation. Only 1 schizont was seen in Aotus hepatocytes at day 13. The morphology and development rates of P. malariae exoerythrocytic stages obtained in vitro were similar to those previously described in vivo.AS.