Short term in vitro cultivation of malaria parasites in Papua New Guinea for preparation of biosynthetically-labelled parasite antigens
Brown, G.V.; Howard, R.J.; Stace, J.D.; Mitchell, G.F.
Papua and New Guinea Medical Journal 22(4): 37-42
1979
ISSN/ISBN: 0031-1480 PMID: 400462 Document Number: 142114
Cultures of the asexual blood stage of Plasmodium falciparum using the Trager and Jensen candlejar technique were established in a laboratory in Papua New Guinea; there was no flow cabinet or incubator, the candlejar had to be immersed in a water bath. Cultures lasted for up to 48 h. Successful growth was demonstrated by the incorporation of [3H]leucine. Growth of the parasites was also observed after they had been exposed to chloroquine in vivo or in vitro. Parasites taken from a patient who had received chloroquine 45 min previously, and parasites cultured in medium containing chloroquine, grew normally to the late trophozoite stage only.