Plasminogen activator (PA) in Guerin epithelioma. Additional PA inhibitor in plasma of rats bearing the epithelioma
Tomasiuk, R.; Mirowski, M.; Wierzbicki, R.
Neoplasma 37(5): 511-519
1990
ISSN/ISBN: 0028-2685 PMID: 2146518 Document Number: 356548
The presence of plasminogen activator (PA) with a Mr of about 35,000 was detected by SDS-PAGE in extract from Guerin epithelioma. The activator, which is a serine proteinase, was partially purified on Sephadex G-50 followed by Lys-Sepharose. Rat plasma, both of healthy and epithelioma-bearing animals, inhibited the activity of such isolated PA. However, the difference between these plasmas in their antiactivator action was observed after inactivation of plasma proteinase inhibitors. In such conditions, the control plasma lost the ability to inhibit the examined PA, whereas the plasma of epithelioma bearing rats retained this ability.