Top Abstract Purchase Related
Home
>
Node
>
Subnode

The cytoskeleton of stromal cells from human bone marrow cultures resembles that of cultured smooth muscle cells

Charbord, P.; Lerat, H.; Newton, I.; Tamayo, E.; Gown, A.M.; Singer, J.W.; Herve, P.

Experimental Hematology 18(4): 276-282

1990

ISSN/ISBN: 0301-472X
PMID: 2182332
Document Number: 355888
The cytoskeleton of stromal cells from the adherent layer of human Dexter-type cultures has been studied. It was found that the stress fibers contained actin specific for smooth muscle, mainly the .alpha.SM actin isoform. The intermediate filaments consisted of vimentin, and there were no desmin filaments. This pattern was similar to that of cultured vascular smooth muscle cells. The detectability of the .alpha.SM actin isoform is coincident with the appearance of stromal cells in long-term marrow cultures and may provide a useful marker for stromal cells. The potential in vivo cellular counterpart for stromal cells generated in the Dexter-type culture system is discussed.

Document emailed within 1 workday
Secure & encrypted payments

Zorina, A.I.; Gorskaia, I.F.; Grosheva, A.G.; Fridenshteĭn, A.I. 1988: Effect of bone marrow cells on colony-forming stromal cells in guinea pigs and on the proliferation of their cultured descendents Biulleten' Eksperimental'noi Biologii i Meditsiny 106(12): 716-718
Zhang, K.-j.; Zhu, Y.; Sun, H.-y.; Huang, L.-f.; Fu, J.-r.; Liu, W.-l. 2006: Mechanism of radiation induced premature senescence of bone marrow stromal cells: experiment with murine bone marrow stromal cells Zhonghua Yi Xue Za Zhi 86(48): 3431-3434
Sasaguri, Y.; Morimatsu, M.; Nakashima, T.; Tokunaga, O.; Yagi, K. 1985: Difference in the inhibitory effect of linoleic acid hydroperoxide on prostacyclin biosynthesis between cultured endothelial cells from human umbilical cord vein and cultured smooth muscle cells from rabbit aorta Biochemistry International 11(4): 517-521
Kharlamova, L.A.; Kolesnikov, A.I.; Vorob'ev, A.I. 1975: Changes in the quantity and level of bone marrow cells and precursor cells of granulopoiesis in organ cultures of human bone marrow Problemy Gematologii i Perelivaniia Krovi 20(12): 45-49
Fridenshteĭn, A.I.; Latsinik, N.V.; Gorskaia, I.F.; Luriia, E.A. 1990: The humoral nature of the colony-stimulating action of bone marrow cells on stromal colony formation in bone marrow cultures Biulleten' Eksperimental'noi Biologii i Meditsiny 110(11): 509-510
Han, J.; Chen, R.; Chen, H.; Deng, L.; Tang, F.; Tian, W. 2001: Growth and osteogenesis characteristics of cultured rat marrow stromal cells under bone induction condition Hua Xi Yi Ke da Xue Xue Bao 32(2): 235-239
Santini, D.; Ceccarelli, C.; Leone, O.; Pasquinelli, G.; Piana, S.; Marabini, A.; Martinelli, G.N. 1995: Smooth muscle differentiation in normal human ovaries, ovarian stromal hyperplasia and ovarian granulosa-stromal cells tumors Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology Inc 8(1): 25-30
Seki, J.; Koyama, N.; Kovach, N.L.; Yednock, T.; Clowes, A.W.; Harlan, J.M. 1996: Regulation of beta1-integrin function in cultured human vascular smooth muscle cells Circulation Research 78(4): 596-605
James, A.F.; Okada, T.; Horie, M. 1995: A fast transient outward current in cultured cells from human pulmonary artery smooth muscle American Journal of Physiology 268(6 Pt 2): H2358-H2365
Resink, T.J.; Scott-Burden, T.; Boulanger, C.; Weber, E.; Bühler, F.R. 1990: Internalization of endothelin by cultured human vascular smooth muscle cells: characterization and physiological significance Molecular Pharmacology 38(2): 244-252
Lin, C-long.; Zhang, Z-xiang.; Xu, Y-jian.; Ni, W.; Chen, S-xin. 2005: Focal adhesion kinase antisense oligodeoxynucleotides inhibit human pulmonary artery smooth muscle cells proliferation and promote human pulmonary artery smooth muscle cells apoptosis Chinese Medical Journal 118(1): 20-26
O'Reilly, G.; Charnock-Jones, D.S.; Cameron, I.T.; Smith, S.K.; Davenport, A.P. 1993: Endothelin-2 mRNA splice variants detected by RT-PCR in cultured human vascular smooth muscle and endothelial cells Journal of Cardiovascular Pharmacology 22(Suppl): S18-S21
Astakhova, V.s. 1987: Stability of stromal precursor cells of human bone marrow preserved under various conditions Gematologiia i Transfuziologiia 32(8): 40-43
Zhang, W.; Liu, D.; Wo, X.; Zhang, Y.; Jin, M.; Ding, Z. 1999: Effects of Curcuma Longa on proliferation of cultured bovine smooth muscle cells and on expression of low density lipoprotein receptor in cells Chinese Medical Journal 112(4): 308-311
Shintani, A.; Nakao, N.; Kakishita, K.; Itakura, T. 2007: Bone marrow stromal cell-mediated enhancement in the survival of dopamine cells and neuronal differentiation from ES cells No Shinkei Geka. Neurological Surgery 35(6): 607-614
Notarnicola, A.; Vicenti, G.; Maccagnano, G.; Silvestris, F.; Cafforio, P.; Moretti, B. 2016: Extracorporeal shock waves induce osteogenic differentiation of human bone-marrow stromal cells Journal of Biological Regulators and Homeostatic Agents 30(4 Suppl 1: 139-144
Kolesnikova, A.I.; Khoptynskaia, S.K.; Konopliannikov, A.G.; Pavlov, V.V.; Kaplan, V.P. 1985: The number of stromal and granulocyte and macrophage precursor cells in human and animal bone marrow before and after splenectomy Gematologiia i Transfuziologiia 30(10): 31-33
Kocher, O.; Skalli, O.; Bloom, W.S.; Gabbiani, G. 1984: Cytoskeleton of rat aortic smooth muscle cells. Normal conditions and experimental intimal thickening Laboratory Investigation; a Journal of Technical Methods and Pathology 50(6): 645-652
Peled, A.; Lee, B.C.; Sternberg, D.; Toledo, J.; Aracil, M.; Zipori, D. 1996: Interactions between leukemia cells and bone marrow stromal cells: stroma-supported growth vs. serum dependence and the roles of TGF-beta and M-CSF Experimental Hematology 24(6): 728-737
Ramalho, A.Claudia.; Couttet, P.; Baudoin, C.; Morieux, C.; Graulet, A-Marie.; de Vernejoul, M-Christine.; Cohen-Solal, M.E. 2002: Estradiol and raloxifene decrease the formation of multinucleate cells in human bone marrow cultures European Cytokine Network 13(1): 39-45