The humoral nature of the colony-stimulating action of bone marrow cells on stromal colony formation in bone marrow cultures
Fridenshteĭn, A.I.; Latsinik, N.V.; Gorskaia, I.F.; Luriia, E.A.
Biulleten' Eksperimental'noi Biologii i Meditsiny 110(11): 509-510
1990
ISSN/ISBN: 0365-9615 PMID: 2083334 Document Number: 349304
In 24 hours adherent marrow cell cultures (AMCC) were represented by single stretched fibroblasts. In non-feeder-supplemented AMCC most of the CFU-f remained single fibroblasts or passed through 1-3 cell doublings . The colony stimulating activity of irradiated marrow cells was found to be diffuse across the Millipore filter, which seems to indicate that haemopoietic marrow cells produce a colony stimulating factor which is required for triggering the CFU-f from the Go-period of the cell cycle into cell proliferation.