Isolation, purification and immunological evaluation of toxin Hb from scorpion Heterometrus bengalensis (C.L. Koch) venom
Dasgupta, S.C.; Gomes, A.; Gomes, A.; Basu, A.; Lahiri, S.C.
Indian Journal of Experimental Biology 28(2): 144-148
1990
ISSN/ISBN: 0019-5189 PMID: 2370074 Document Number: 350895
Toxin Hb, a lethal toxic antigenic protein, isolated from the venom of Heterometrus bengalensis by CM-cellulose ion-exchange chromatography was a heat labile basic protein with a molecular weight of 10 kDa. It produced irreversible blockade on the isolated rat phrenic nerve diaphragm and chick biventer cervicis. LD50 of toxin Hb was 0.48 mg/kg (iv) in mice. Antiserum was raised in mice by hyperimmunization against toxin Hb. Antitoxin Hb antiserum was immunologically potent as revealed by immunogel-diffusion and immunoelectrophoresis. Five fold protection against the lethal action of toxin Hb was achieved by the antiserum. It also effectively antagonised toxin Hb induced neuromuscular blockade on isolated rat phrenic nerve diaphragm and chick biventer cervicis preparations.[H. bengalensis is common is eastern India, particularly in West Bengal; its sting is occasionally fatal for children.]AS Toxin Hb, isolated from the venom of H. bengalensis by CM-cellulose ion-exchange chromatography, is a heat labile basic protein with a molecular weight of 10 kDa. It produced an irreversible blockade on isolated rat phrenic nerve diaphragm and chick biventer cervicis preparations. The LD50 of toxin Hb was 0.48 mg/kg in mice. Antiserum was raised in mice by hyperimmunization against toxin Hb. Antitoxin Hb antiserum was immunologically potent as revealed by immunodiffusion tests and immunoelectrophoresis. 5-fold protection against the lethal action of toxin Hb was achieved by the antiserum. It also effectively antagonised toxin Hb induced neuromuscular blockade on isolated rat phrenic nerve diaphragm and chick biventer cervicis preparations.