Kinetic properties of 5'-nucleotidase in the adipose tissue, liver and blood of rats of different ages
Egutkin, G.G.; Iakubovskiĭ, S.M.; Gatsko, G.G.
Ukrainskii Biokhimicheskii Zhurnal 62(3): 95-98
1990
ISSN/ISBN: 0201-8470 PMID: 2396326 Document Number: 350132
The kinetic characteristics (Vmax and Km) of membrane-bound and serum 5'-nucleotidases from rats of different age groups are studied. The age-related increase of Km parameter in plasma membranes of fatty tissue and liver demonstrated a lower enzyme affinity to the substrate in ageing membranes. The specific activity of 5'-nucleotidase in blood serum of aged animals was below that in young ones, while Km value remained stable. Tissue differentiation of the catalytic properties of the enzyme and their aged-related characteristics may be to a considerable extent determined by the physicochemical state of cell membranes.