Use of LeuM1 monoclonal antibody for the diagnosis of Hodgkin's disease
Memon, G.M.; Alam, S.M.
Jpma. Journal of the Pakistan Medical Association 40(7): 156-159
1990
ISSN/ISBN: 0030-9982 PMID: 1980316 Document Number: 350080
Monoclonal antibody to LeuM1, a granulocyte-related differentiation antigen, represents a highly effective reagent for detection of diagnostic "Reed-Sternberg" cells and variants in paraffin-embedded tissues of Hodgkin's disease. The "Reed Sternberg" cell in all the cases of Hodgkin's disease except lymphocyte predominance variety revealed positive intracytoplasmic/paranuclear granular staining with LeuM1 marker. The R-S cells in lymphocyte predominance variety contain probably sialylated LeuM1 antigen. All the cases of non-Hodgkin's lymphoma and reactive lymphadenitis showed no staining with LeuM1 monoclonal antibody. Therefore this antibody represents a potentially helpful diagnostic discriminant in the assessment of Hodgkin's disease and its distinction from non-Hodgkin's lymphomas and morphologically similar reactive lymphoid lesions.