A monoclonal antibody to an antigen present in cells from a patient with Hodgkin's disease
Veenstra, H.; Close, P.; Dowdle, E.B.
South African Medical Journal 72(11): 794-796
1987
ISSN/ISBN: 0256-9574 PMID: 3317934 Document Number: 297925
A monoclonal antibody-secreting hybridoma cell line, VCD-1, was derived from the fusion of murine myeloma cells with splenocytes from a BALB/c mouse that had been immunized with chronic B-lymphocytic leukemia cells. The cells came from a patient who had developed the leukemia approximately 10 years after a course of radiotherapy for nodular sclerosing Hodgkin's disease. The antibody bound to a 30,000-dalton protein that was present in normal and malignant B cells, in monocytes, neutrophils, and interdigitating reticulum cells, and in malignant cells present in Hodgkin's disease lymph nodes. The reactive epitope was not accessible to antibody in viable intact cells; binding to peripheral blood cells could only be seen if the cells were fixed. The antibody recognizes a determinant that probably residues on the .alpha.-chain of HLA class II molecules.