Effects of galanin on electrical activity of pancreatic islet cells
Fu, X.W.; Qian, Z.W.; Zhang, J.R.
Zhongguo Yao Li Xue Bao 11(4): 350-354
1990
ISSN/ISBN: 0253-9756 PMID: 1716812 Document Number: 348263
Intracellular microelectrode technique was used to investigate the effect of galanin on the electrical activity of .beta. cells of Langerhans islets of mice. Galanin 0.15 and 0.3 .mu.mol/L in the perfusion medium inhibited the basic electrical activity induced by glucose 5.5, 11.1 and 20 mmol/L in decreasing the frequency and amplitude of the spikes. In some cells, during galanin inhibitors there was a hyperpolarization after the spike. Verapamil (30, 60 and 90 .mu.mol/L), a blocker of voltage-dependent Ca channels, blocked dose-dependently the electrical activity induced by glucose and attenuated the depolarization induced by KCl 50 mmol/L. Galanin 0.3 .mu.mol/L also attenuated the depolarization induced by KCl 50 mmol/L, similar to the effect of verapamil. The results suggest that the effect of galanin on inhibition of the electrical activity of .beta. cell might be due to blocking of voltage-dependent Ca2+ channels.