Increase in the in vitro activity of mollicute transcriptase using substances of an organic nature
Skripal', I.G.; Babichev, V.V.
Mikrobiolohichnyi Zhurnal 51(3): 19-23
1989
ISSN/ISBN: 0201-8462 PMID: 2796783 Document Number: 343582
It is established that the use of DNA-dependent RNA-polymerases of mollicutes in the molecular-biological and gene-engineering works aimed to obtain a sufficient amount of the transcription products may promote a 1.5-2-fold increase of the yield by introducing such substances as glycerol, dimethylsulphoxide and spermidine (optimal concentrations: 10-12 vol. % and 12 mM, respectively) into the composition of the reacting mixture.
Document emailed within 1 workday
Related Documents
Cavrini, V.; Gatti, R.; Giovanninetti, G.; Chiarini, A.; Franchi, L.; Landini, M.P. 1980: Substances with antiviral activity. XIII. Synthesis and in vitro activity of thiosemicarbazones of 1-benzoyl- and 1-benzyl-2-chloro-3-formylindoles Il Farmaco; Edizione Scientifica 35(8): 636-641Adjobimey, T.; Edaye, I.; Lagnika, L.; Gbenou, J.; Moudachirou, M.; Sanni, A. 2004: Activités antiplasmodiales in vitro de quelques plantes antipaludiques de la pharmacopée béninoise : Les substances chimiques biologiquement actives extraites des plantes médicinales et les huiles essentielles des plantes aromatiques de la sous-région Ouest-Africaine - In vitro antiplasmodial activity of some antimalarial plants of beninese pharmacopoeia : Biologically active substances extracted from medicinal plants and essential oils from aromatic plants of West Africa Comptes Rendus. Chimie 7(10-11): 1023-1027
Adaikan, P.G.; Zaman, V. 1982: In vitro activity of anthelmintic drugs and biologically active substances on Breinlia booliati Southeast Asian Journal of Tropical Medicine and Public Health 13(2): 257-261
Von Ardenne, M.; Klemm, W. 1987: Measurements of the increase in the difference between the arterial and venous Hb-O2 saturation obtained with daily administration of 200 mg standardized ginseng extract G115 for four weeks. Long-term increase of the O2 transport into the organs and tissues of the organism through biologically active substances Panminerva Medica 29(2): 143-150
Romanenko, V.I.; Kuznetsov, S.I. 1972: Decomposition of organic substances in bottom deposits Mikrobiologiia 41(2): 356-361
Zverev, I.F. 1978: Effect of dichlothiazide on the transport of organic substances in the kidneys Farmakologiia i Toksikologiia 41(1): 69-72
Gulaia, N.K.; Tiuten'kova, N.L. 1974: Destruction of organic substances in the soil of the Kapchagai reservoir Mikrobiologiia 43(2): 316-320
Konyshev, V.A. 1976: Nature of substances regulating cell proliferation processes in animals Tsitologiia 18(9): 1047-1058
Dmitriev, M.T.; Kitrosskiĭ, N.A.; Maslenkovskiĭ, L.G. 1976: Gas chromatographic determination of total concentration of organic substances using air as a gas-bearer Gigiena i Sanitariia 11: 79-82
Malysheva, A.G.; Rastiannikov, E.G. 1993: Determination of volatile organic substances in soil by mass chromatography spectrometry Gigiena i Sanitariia 5: 66-68
Crane, G.A.; Mittleman, M.W.; Stephan, M. 1991: Total organic carbon measurement as a substitute for the USP oxidizable substances test Journal of Parenteral Science and Technology: a Publication of the Parenteral Drug Association 45(1): 20-28
Chernitsyna, M.A.; Braun, D.D. 1986: Use of gas chromatography for determining the migration of organic substances from polyolefins intended for use in the food industry Gigiena i Sanitariia 6: 54-57
Hatch, R.C.; Laflamme, D.P. 1989: Acute intraperitoneal cholecalciferol (vitamin D3) toxicosis in mice: its nature and treatment with diverse substances Veterinary and Human Toxicology 31(2): 105-112
Ricchetti, M.; Buc, H. 1997: Telomerase activity of reverse transcriptase Science 277(5328): 887
Sager, G. 1978: Integrations of increase functions according to the L. V. Bertalanffy conception of organic growth Anatomischer Anzeiger 144(2): 147-157
Martyniuk, V.K.; Fedorov, A.A.; Kurochkin, V.I.; Volkova, N.A.; Okunev, P.V.; Zhernakova, Z.M.; Chudinova, O.A.; Roslyĭ, O.F.; Guliaev, V.I.; Potapov, L.V. 2005: Experimental base for use of low-mineral water with a high concentration of organic substances in balneological practice Voprosy Kurortologii Fizioterapii i Lechebnoi Fizicheskoi Kultury 5: 8-11
Grushko, I.M. 1971: Toxic substances of organic origin in industrial waters and the problem of sanitary protection of reservoirs Gigiena i Sanitariia 36(10): 82-85
Shapovalova, O.V.; Dolgova, G.V.; Neugodova, N.P.; Sapozhnikova, G.A. 2013: Organic solvents for determination of bacterial endotoxin index in water-insoluble pharmaceutical substances Antibiotiki i Khimioterapiia 58(9-10): 41-44
Kantorovich, E.N.; Berezina, O.N.; Moisiadi, S.A.; Bektemirov, T.A. 1975: RNA transcriptase activity of a defective influenza virus Voprosy Virusologii 1975(2): 242-243
Novikov, V.S.; Bortnovskiĭ, V.N.; Mastriukov, A.A.; Lotovin, A.P.; Arzumanov, A.A. 1987: Efficacy of the use of biologically active substances to increase the body resistance in sailors Voenno-Meditsinskii Zhurnal 10: 50-51