Methyl-cytosine specific restriction in Escherichia coli K-12
Povilenis, P.I.; Lubis, A.A.; Vaĭshvila, R.I.; Kulakauskas, S.T.; Ianulaĭtis, A.A.
Genetika 25(4): 753-755
1989
ISSN/ISBN: 0016-6758 PMID: 2547699 Document Number: 341935
Experiments on transformation of Escherichia coli K-12 cells by plasmids carrying RM systems with different recognition sites containing 5-methylcytosine have shown that the gene mcrB determines the function of restriction. The data obtained made it possible to believe that E. coli possesses no restriction system recognizing specifically cytosine methylated in position 4.
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