The effect of metabolic inhibitors on resistance of mannose-specific contacts of Escherichia coli K12 and human neutrophils
Timoshenko, A.V.; Fomichev, A.I.; Cherenkevich, S.N.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 5: 9-13
1994
ISSN/ISBN: 0208-0613 PMID: 7891733 Document Number: 422577
Human neutrophil aggregation induced by Escherichia coli K12 cells has been studied. D-mannose has been found to inhibit the process in the dose-dependent way causing the full blockage at 10 mM concentration. The process of disaggregation induced by the concentration is significantly higher at 10 degrees C than at 37 degrees C. The inhibitors of cellular oxygenation such as N-ethylmaleimide, nordihydroguaiaretic acid, trifluoperazine, and colchicine did not affect the aggregation process. Cellular aggregation was concomitant with production of H2O2, and this reaction was also blocked by D-mannose and depended on the number of bacteria present. The nordihydroguaiaretic acid and N-ethylmaleimide inhibited production of H2O2, while sodium azide enhanced the process. The results show the mannose-specific contact of bacteria and neutrophils to depend on the functional activity of phagocytes including their ability to produce H2O2.