Top Abstract Purchase Related
Home
>
Node
>
Subnode

Isolation and properties of DNA-cytosine-methylase i from Escherichia coli MRE 600

Nesterenko, V.F.; Bur'ianov, I.I.; Baev, A.A.

Biokhimiia 44(1): 130-141

1979

ISSN/ISBN: 0320-9725
PMID: 369620
Document Number: 153085
DNA-cytosine-methylase I was isolated and purified to homogeneity. The yield made up to about 30% of total activity. The enzyme molecular weight as determined by centrifugation in a sucrose gradient, by gel filtration and by electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulfate was found to be 45,000. The Michaelis constant was 1,8 . 10(-6) M for SAM and 2 . 10(-4) M for DNA. DNA-cytosine-methylase I modifies phage lambda DNA in 60 sites. This modification does not protect DNA from the effects of restriction endonucleases HpaII and BsuRI. The enzyme methylates DNA in the nucleotide sequence: 5'...Pur-MC-C-G-G-Pyr...3'.

Document emailed within 1 workday
Secure & encrypted payments

Bogdarina, I.G.; Bur'ianov, I.I.; Baev, A.A. 1979: Isolation and properties of DNA-cytosine methyltransferase from Escherichia coli C Molekuliarnaia Biologiia 13(2): 281-291
Tseng, C.C. 1981: Studies on pathogenic Escherichia coli. Part 8. Purification and properties of exotoxin from capsular polysaccharide-synthesizing Escherichia coli Journal of the Formosan Medical Association 80(9): 866-872
Aĭzenberg, O.A.; Torosian, M.V.; Naumova, L.A.; Rabinkova, E.V.; Fradkin, G.E. 1986: Effect of mutation in DNA unwinding protein on the viability, UV- and gamma-sensitivity of Escherichia coli K12 mutants defective for DNA-polymerase i and DNA-methylase Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 3: 34-42
Povilenis, P.I.; Lubis, A.A.; Vaĭshvila, R.I.; Kulakauskas, S.T.; Ianulaĭtis, A.A. 1989: Methyl-cytosine specific restriction in Escherichia coli K-12 Genetika 25(4): 753-755
Zell, R.; Fritz, H.J. 1987: DNA mismatch-repair in Escherichia coli counteracting the hydrolytic deamination of 5-methyl-cytosine residues EMBO Journal 6(6): 1809-1815
Li, Z.; Shanmugam, N.; Katayose, D.; Huber, B.; Srivastava, S.; Cowan, K.; Seth, P. 1997: Enzyme/prodrug gene therapy approach for breast cancer using a recombinant adenovirus expressing Escherichia coli cytosine deaminase Cancer Gene Therapy 4(2): 113-117
Moorthy, A.R. 1985: Isolation of Escherichia coli from an aborted bovine fetus Veterinary Record 116(6): 159
Pérez, P.F.; Constanza, O.; Garré, M.; Morelli, I.; Martínez, A.; Vecchioli, G.; Painceira, M.T. 1993: Isolation of antibiotic-resistant Escherichia coli from the water of the Río de la Plata Revista Argentina de Microbiologia 25(1): 7-14
Radnedge, L.; Pinney, R.J. 1993: Post-UV survival and mutagenesis in DNA repair-proficient and -deficient strains of Escherichia coli K-12 grown in 5-azacytidine to inhibit DNA cytosine methylation: evidence for mutagenic excision repair Journal of Pharmacy and Pharmacology 45(3): 192-197
De Biase, D.; Simmaco, M.; Sweeney, G.; Barra, D.; Bossa, F.; John, R.A. 1993: Isolation and cloning of the gene from Escherichia coli encoding glutamate decarboxylase Biotechnology and Applied Biochemistry 18(2): 139-142
Svetlikova, S.B.; Kozlov, A.V. 1978: Isolation of ribosomes from Escherichia coli by chromatography on methyl-albumin-kieselgel Acta Biologica et Medica Germanica 37(9): 1343-1352
Orozco de Silva, A.; Toro, S. 1990: Isolation and characterization of mutants affected in the expression of the nar operon Escherichia coli Acta Cientifica Venezolana 41(1): 21-25
Fischer, B.; Sumner, I.; Goodenough, P. 1992: Isolation and renaturation of bio-active proteins expressed in Escherichia coli as inclusion bodies Arzneimittel-Forschung 42(12): 1512-1515
Moon, H.W.; Rogers, D.G.; Rose, R. 1988: Effects of an orally administered live Escherichia coli pilus vaccine on duration of lacteal immunity to enterotoxigenic Escherichia coli in swine American Journal of Veterinary Research 49(12): 2068-2071
Ruiz-Herrera, J.; Ramiŕez, A.M. 1973: Solubilization and properties of succinate dehydrogenase from Escherichia coli Revista Latinoamericana de Microbiologia 15(3): 133-138
Martin, U.; Fischer, S.; Kohnert, U.; Lill, H.; Rudolph, R.; Sponer, G.; Stern, A.; Strein, K. 1990: Properties of a novel plasminogen activator (BM 06.022) produced in Escherichia coli Zeitschrift für Kardiologie 79(Suppl 3): 167-170
Vernozy-Rozand, C. 1999: Verotoxin-producing Escherichia coli (VTEC) and Escherichia coli O157:H7 in medicine and food industry Annales de Biologie Clinique 57(5): 507-515
Martin, M.L.; Shipman, L.D.; Wells, J.G.; Potter, M.E.; Hedberg, K.; Wachsmuth, I.K.; Tauxe, R.V.; Davis, J.P.; Arnoldi, J.; Tilleli, J. 1986: Isolation of Escherichia coli O157:H7 from dairy cattle associated with two cases of haemolytic uraemic syndrome Lancet 2(8514): 1043
Villaseca, J.M.; Hernández, U.; Sainz-Espuñes, T.R.; Rosario, C.; Eslava, C. 2005: Enteroaggregative Escherichia coli an emergent pathogen with different virulence properties Revista Latinoamericana de Microbiologia 47(3-4): 140-159
Romano Carratelli, C.; Nuzzo, I.; Galdiero, M. 1987: Surface properties of Escherichia coli strains responsible for urinary infections Microbiologica 10(1): 55-61