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Isolation of ribosomes from Escherichia coli by chromatography on methyl-albumin-kieselgel

Svetlikova, S.B.; Kozlov, A.V.

Acta Biologica et Medica Germanica 37(9): 1343-1352

1978

ISSN/ISBN: 0001-5318
PMID: 373347
Document Number: 133524
The present communication shows that ribosomes isolated by differential centrifugation retain their composition and biological activity during subsequent methyl-albumin-kieselgel (MAK)-chromatography. The data obtained were used to develop a novel chromatographic procedure for the isolation of ribosomes from E. coli lysates. It could be demonstrated that purified preparations of active ribosomes can be obtained by MAK-chromatography avoiding ultracentrifugation.

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Kurek, E.; Grankowski, N.; Gasior, E. 1972: On the phosphorylation of Escherichia coli ribosomes. I. An in vivo labeling of ribosomes Acta Microbiologica Polonica. Series A: Microbiologia Generalis 4(4): 171-176
Sumpter, V.G.; Trotman, C.N.; Tate, W.P. 1985: Modification of Escherichia coli ribosomes: in vitro termination is less dependent on histidine residues at the peptidyl transferase centre when ribosomes lack protein L11 Biochemistry International 10(2): 137-146
Babkina, G.T.; Vladimirov, S.N.; Dryga, S.A.; Zenkova, M.A.; Karpova, G.G. 1988: MRNA-binding site of ribosomes at different stages of translation. II. Affinity modification of Escherichia coli ribosomes bya benzylidene derivative of AUGU6 in pre- and post-translation complexes Molekuliarnaia Biologiia 22(2): 357-361
Bogdanov, A.A. 1978: Role of ribonucleic acids in organizing the structure of Escherichia coli ribosomes Molekuliarnaia Biologiia 12(4): 725-736
Lavrik, I.N.; Sergiev, P.V.; Bogdanov, A.A.; Zimmermann, R.A.; Dontsov, O.A. 2004: Escherichia coli ribosomes as the model to test new photoactivated tRNA analogues, containing 6-thioguanosine Molekuliarnaia Biologiia 38(5): 937-944
Chomczyński, P.; Szafrański, P. 1971: The effect of high salt concentration on fidelity of translation by Escherichia coli ribosomes Acta Biochimica Polonica 18(2): 163-170
Dey, D.N.; Burma, D.P. 1991: Polyclonal antibodies as probes to distinguish between tight and loose couple 50S ribosomes of Escherichia coli Indian Journal of Biochemistry and Biophysics 28(5-6): 369-373
Nekhaĭ, S.A.; Saminskiĭ, E.M. 1994: Study of the poly(U)-dependent interaction of tRNA(Phe) with the P-site of Escherichia coli ribosomes by chemical modification with nitrosoethylurea Molekuliarnaia Biologiia 28(4): 926-931
Budker, V.G.; Grineva, N.I.; Karpova, G.G.; Kobets, N.D.; Maev, S.P. 1977: Affinity modification of Escherichia coli ribosomes in the region of the mRNA-binding center by a heptauridylate analog bearing a chemically active group at the 3' end Molekuliarnaia Biologiia 12(3): 539-545
Povilenis, P.I.; Lubis, A.A.; Vaĭshvila, R.I.; Kulakauskas, S.T.; Ianulaĭtis, A.A. 1989: Methyl-cytosine specific restriction in Escherichia coli K-12 Genetika 25(4): 753-755
Hui, A.; Jhurani, P.; de Boer, H.A. 1987: Directing ribosomes to a single mRNA species: a method to study ribosomal RNA mutations and their effects on translation of a single messenger in Escherichia coli Methods in Enzymology 153: 432-452
Katunin, V.I.; Kirillov, S.V. 1984: Binding of the yeast phenylalanine tRNA with Escherichia coli ribosomes. Effect of the removal of a modified base from the 3'-end of the anticodon on codon-anticodon interaction Molekuliarnaia Biologiia 18(6): 1486-1496
Nesterenko, V.F.; Bur'ianov, I.I.; Baev, A.A. 1979: Isolation and properties of DNA-cytosine-methylase i from Escherichia coli MRE 600 Biokhimiia 44(1): 130-141
Moorthy, A.R. 1985: Isolation of Escherichia coli from an aborted bovine fetus Veterinary Record 116(6): 159
Bogdarina, I.G.; Bur'ianov, I.I.; Baev, A.A. 1979: Isolation and properties of DNA-cytosine methyltransferase from Escherichia coli C Molekuliarnaia Biologiia 13(2): 281-291
Zell, R.; Fritz, H.J. 1987: DNA mismatch-repair in Escherichia coli counteracting the hydrolytic deamination of 5-methyl-cytosine residues EMBO Journal 6(6): 1809-1815
Pérez, P.F.; Constanza, O.; Garré, M.; Morelli, I.; Martínez, A.; Vecchioli, G.; Painceira, M.T. 1993: Isolation of antibiotic-resistant Escherichia coli from the water of the Río de la Plata Revista Argentina de Microbiologia 25(1): 7-14
Tsvetkova, N.A.; Goliasnaia, N.V. 2007: Induction of the SOS response in Escherichia coli cells under osmotic stress and in the presence of N-methyl-N'-nitro-N-nitrosoguanidine Mikrobiologiia 76(4): 448-455
De Biase, D.; Simmaco, M.; Sweeney, G.; Barra, D.; Bossa, F.; John, R.A. 1993: Isolation and cloning of the gene from Escherichia coli encoding glutamate decarboxylase Biotechnology and Applied Biochemistry 18(2): 139-142
Orozco de Silva, A.; Toro, S. 1990: Isolation and characterization of mutants affected in the expression of the nar operon Escherichia coli Acta Cientifica Venezolana 41(1): 21-25