Top Abstract Purchase Related
Home
>
Node
>
Subnode

Polyclonal antibodies as probes to distinguish between tight and loose couple 50S ribosomes of Escherichia coli

Dey, D.N.; Burma, D.P.

Indian Journal of Biochemistry and Biophysics 28(5-6): 369-373

1991

ISSN/ISBN: 0301-1208
PMID: 1812069
Document Number: 387680
Antibody has been raised in rabbit against L7/L12 protein of E. coli 50S ribosomes and purified, finally through affinity column. A sensitive assay method using ELISA technique has also been standardised. LC 50S ribosomes react more with the antibody than TC 50S ribosomes. This supports the earlier physical data [Burma D P, Srivastava A K, Srivastava S, Tewari D S, Dash D & Sengupta S K, (1984), Biochem Biophys Res Commun, 124, 970] indicating that L7/L12 stalk region is protruded in medium in LC ribosomes and folded towards the body in TC ribosomes.

Document emailed within 1 workday
Secure & encrypted payments

Kurek, E.; Grankowski, N.; Gasior, E. 1972: On the phosphorylation of Escherichia coli ribosomes. I. An in vivo labeling of ribosomes Acta Microbiologica Polonica. Series A: Microbiologia Generalis 4(4): 171-176
Sumpter, V.G.; Trotman, C.N.; Tate, W.P. 1985: Modification of Escherichia coli ribosomes: in vitro termination is less dependent on histidine residues at the peptidyl transferase centre when ribosomes lack protein L11 Biochemistry International 10(2): 137-146
Babkina, G.T.; Vladimirov, S.N.; Dryga, S.A.; Zenkova, M.A.; Karpova, G.G. 1988: MRNA-binding site of ribosomes at different stages of translation. II. Affinity modification of Escherichia coli ribosomes bya benzylidene derivative of AUGU6 in pre- and post-translation complexes Molekuliarnaia Biologiia 22(2): 357-361
Thomas, P.E.; Bandiera, S.; Reik, L.M.; Maines, S.L.; Ryan, D.E.; Levin, W. 1987: Polyclonal and monoclonal antibodies as probes of rat hepatic cytochrome P-450 isozymes Federation Proceedings 46(8): 2563-2566
Svetlikova, S.B.; Kozlov, A.V. 1978: Isolation of ribosomes from Escherichia coli by chromatography on methyl-albumin-kieselgel Acta Biologica et Medica Germanica 37(9): 1343-1352
Bogdanov, A.A. 1978: Role of ribonucleic acids in organizing the structure of Escherichia coli ribosomes Molekuliarnaia Biologiia 12(4): 725-736
Chomczyński, P.; Szafrański, P. 1971: The effect of high salt concentration on fidelity of translation by Escherichia coli ribosomes Acta Biochimica Polonica 18(2): 163-170
Lavrik, I.N.; Sergiev, P.V.; Bogdanov, A.A.; Zimmermann, R.A.; Dontsov, O.A. 2004: Escherichia coli ribosomes as the model to test new photoactivated tRNA analogues, containing 6-thioguanosine Molekuliarnaia Biologiia 38(5): 937-944
Nekhaĭ, S.A.; Saminskiĭ, E.M. 1994: Study of the poly(U)-dependent interaction of tRNA(Phe) with the P-site of Escherichia coli ribosomes by chemical modification with nitrosoethylurea Molekuliarnaia Biologiia 28(4): 926-931
Echeverria, P.; Seriwatana, J.; Chityothin, O.; Chaicumpa, W.; Tirapat, C. 1982: Detection of enterotoxigenic Escherichia coli in water by filter hybridization with three enterotoxin gene probes Journal of Clinical Microbiology 16(6): 1086-1090
Budker, V.G.; Grineva, N.I.; Karpova, G.G.; Kobets, N.D.; Maev, S.P. 1977: Affinity modification of Escherichia coli ribosomes in the region of the mRNA-binding center by a heptauridylate analog bearing a chemically active group at the 3' end Molekuliarnaia Biologiia 12(3): 539-545
Katunin, V.I.; Kirillov, S.V. 1984: Binding of the yeast phenylalanine tRNA with Escherichia coli ribosomes. Effect of the removal of a modified base from the 3'-end of the anticodon on codon-anticodon interaction Molekuliarnaia Biologiia 18(6): 1486-1496
Hui, A.; Jhurani, P.; de Boer, H.A. 1987: Directing ribosomes to a single mRNA species: a method to study ribosomal RNA mutations and their effects on translation of a single messenger in Escherichia coli Methods in Enzymology 153: 432-452
Tseng, C.C. 1981: Studies on pathogenic Escherichia coli. Part 8. Purification and properties of exotoxin from capsular polysaccharide-synthesizing Escherichia coli Journal of the Formosan Medical Association 80(9): 866-872
Moon, H.W.; Rogers, D.G.; Rose, R. 1988: Effects of an orally administered live Escherichia coli pilus vaccine on duration of lacteal immunity to enterotoxigenic Escherichia coli in swine American Journal of Veterinary Research 49(12): 2068-2071
Vernozy-Rozand, C. 1999: Verotoxin-producing Escherichia coli (VTEC) and Escherichia coli O157:H7 in medicine and food industry Annales de Biologie Clinique 57(5): 507-515
Heddle, R.J.; Shearman, D.J. 1979: Serum antibodies to Escherichia coli in subjects with ulcerative colitis Clinical and Experimental Immunology 38(1): 22-30
Hauser, J. 2006: Quel droit européen en matière de couple ? : Le couple du XXIe siècle - What European law in the matter of couple? The couple in the XXIst century Informations Sociales (129): 58-68
Voravuthikunchai, S.P.; Chaowana, C.; Perepat, P.; Iida, T.; Honda, T. 2005: Antibodies among healthy population of developing countries against enterohaemorrhagic Escherichia coli O157:H7 Journal of Health Population and Nutrition 23(4): 305-310
Voravuthikunchai, S.P.; Chaowana, C.; Perepat, P.; Iida, T.; Honda, T. 2005: Antibodies among healthy population of developing countries against enterohaemorrhagic Escherichia coli O157:H7 Journal of Health Population and Nutrition 23(4): 305-310