Kinetics of cell replication in the uterine cervix. VIII. Quantitation of chronologic events in proliferating loci
Rubio, C.A.; Llatjos, M.; Nilsson, B.
In Vivo 3(6): 363-365
1989
ISSN/ISBN: 0258-851X PMID: 2519879 Document Number: 341581
The frequency and the topographic distribution of DNA replicating cervical basal squamous cells (as deduced by 3H-thymidine in vivo administration up to 8 hours before sacrifice) was studied in 70,000 basal cells in 70 C57B1 mice. While the number of labelled basal cells/animal was similar in those infected only once, a significantly higher number of labelled basal cells was recorded in animals injected twice or three times. Following one single injection, 30% (8 h previouly) and 31% (1 h previously) of the DNA replicating basal cells occurred in pairs or in loci of three, four or more labelled cells. After repreated injections, the rates were 49% for those animals injected twice before sacrifice and 47% for those injected three times. Thus repeated injections resulted in a significantly increased number of loci of three, four or more DNA replicating basal cells when compared to those injected only once. The present results suggest that there is a chronological asynchrony in the DNA replication of proliferating basal cells-loci of the uterine cervix.